![AffiAB® Anti-HNF-4-alpha Antibody [SN72-03]](/web/image/product.product/74693/image_1024/%5BAFG-HB-1909%5D%20AffiAB%C2%AE%20Anti-HNF-4-alpha%20Antibody%20%5BSN72-03%5D%20?unique=a48b62f)
AffiAB® Anti-HNF-4-alpha Antibody [SN72-03]
HNF-4 alpha is a transcription factor that binds DNA as a homodimer. HNF-4 alpha is important in liver, kidney, and intestinal development. It has also been intensely studied as one of a variety of genes responsible for diabetes mellitus. HNF-4 alpha has been shown in knock out mice to be essential for the morphogenic and functional differentiation of hepatocytes. HNF-4 alpha is a dominant regulator of epithelial phenotypes able to drive the mesenchymal-to-epithelial transition when expressed in fibroblasts.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P41235 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SN72-03
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, FC
Predicted species reactivity
Mouse
Immunogen
Synthetic peptide within Human HNF-4-alpha aa 21-70 / 474.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Cardiovascular, AMPK signaling pathway
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:100-1:500; FC: 1:50-1:100
Molecular Weight
Predicted band size: 53 kDa
Subcellular location
Nucleus.
Positive control
A549 cell lysates, LOVO cell lysates, SW480, human colon carcinoma tissue, human kidney tissue, human liver carcinoma tissue, human small intestine tissue, HepG2.