AffiAB® Anti-HLA-DR Antibody
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P01903 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within C-terminal human HLA-DR.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Rheumatoid arthritis, Asthma
Recommended dilutions
WB: 1:1, 000-1:5, 000; IF-Cell: 1:50-1:100; IHC-P: 1:100-1:500; FC: 1:50-1:100
Molecular Weight
Predicted band size: 29 kDa
Subcellular location
Lysosome membrane, Endosome membrane, Late endosome membrane, trans-Golgi network membrane, Endoplasmic reticulum membrane, Cell membrane.
Positive control
Raji cell lysate, Daudi cell lysate, D3, human spleen tissue, Jurkat.