AffiAB® Anti-HKDC1 Antibody
As the recently identified fifth isoform of hexokinase, HKDC1 catalyzes the rate-limiting and first obligatory step of glucose metabolism, which is the ATP-dependent phosphorylation of glucose to G6P. Though its particular biological function remains unclear, HKDC1 has been suggested to play a more major role in glucose metabolism during pregnancy, as the mother would need to provide enough energy for both herself and the fetus. HKDC1 is ubiquitously expressed, with the highest levels of expression in pharynx, thymus, colon, esophagus, and eye tissue.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q2TB90 Human; SwissProt: Q91W97 Mouse; SwissProt: M0R3X7 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human HKDC1 aa 266-462 / 917.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100; WB: 1:500
Molecular Weight
Predicted band size: 82/90/102 kDa
Subcellular location
Cytoplasm, Mitochondrion membrane.
Positive control
LOVO, MCF-7, SiHa, rat kidney tissue, mouse brain tissue.