AffiAB® Anti-Histone H4 (acetyl K5) Antibody [SR31-07]

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CAT# AFG-HB-1877
Size: 100 μL

346.50 346.5 USD 346.50

346.50

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AffiAB® Anti-Histone H4 (acetyl K5) Antibody [SR31-07]

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: P62805 Human; SwissProt: P62806 Mouse; SwissProt: P62804 Rat

Recombinant

YES

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

SR31-07

KO/KD

N/A

Species reactivity

Human, Mouse, Rat

Tested applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, ChIP, CUT&Tag-seq

Predicted species reactivity

N/A

Immunogen

Synthetic peptide within Human Histone H4 aa 1-50 / 103 (acetyl K5) .

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

N/A

Recommended dilutions

WB: 1:1, 000-1:5, 000; IF-Cell: 1:50-1:400; IF-Tissue: 1:50-1:400; IHC-P: 1:50-1:1, 000; IP: Use at an assay dependent concentration.

Molecular Weight

Predicted band size: 11 kDa

Subcellular location

Nucleus, Chromosome.

Positive control

CRC cell lysate, Hela cell lysate, SH-SY5Y cell lysate, Hela, CRC, SH-SY5Y, rat brain tissue, human tonsil tissue, human colon carcinoma tissue, mouse testis tissue, mouse colon tissue, mouse brain tissue.