AffiAB® Anti-Histone H4 (acetyl K5) Antibody [SR31-07]
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P62805 Human; SwissProt: P62806 Mouse; SwissProt: P62804 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SR31-07
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, ChIP, CUT&Tag-seq
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Histone H4 aa 1-50 / 103 (acetyl K5) .
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:5, 000; IF-Cell: 1:50-1:400; IF-Tissue: 1:50-1:400; IHC-P: 1:50-1:1, 000; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 11 kDa
Subcellular location
Nucleus, Chromosome.
Positive control
CRC cell lysate, Hela cell lysate, SH-SY5Y cell lysate, Hela, CRC, SH-SY5Y, rat brain tissue, human tonsil tissue, human colon carcinoma tissue, mouse testis tissue, mouse colon tissue, mouse brain tissue.