![AffiAB® Anti-Histone H4 (acetyl K8) Antibody [PS01-25]](/web/image/product.product/74662/image_1024/%5BAFG-HB-1874%5D%20AffiAB%C2%AE%20Anti-Histone%20H4%20%28acetyl%20K8%29%20Antibody%20%5BPS01-25%5D%20?unique=d31c23b)
AffiAB® Anti-Histone H4 (acetyl K8) Antibody [PS01-25]
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H4K8ac is part of 17 modifications of a group of active promoters. H4K8ac is found more often in active promoters and transcribed regions than other marks. H4K8ac is modified by a different group of enzymes than other H4 lysines.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P62805 Human; SwissProt: P62806 Mouse; SwissProt: P62804 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
PS01-25
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Histone H4 aa 1-20 (acetyl K8) .
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
PBS (pH7.4) , 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000; IF-Cell: 1:50; IHC-P 1:1, 000-1:5, 000
Molecular Weight
Predicted band size: 11 kDa
Subcellular location
Nucleus. Chromosome.
Positive control
Human skin tissue, rat skin tissue, human colon carcinoma tissue, PC-12, Hela cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate.