AffiAB® Anti-Histone H3.1 Antibody
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P68431 Human; SwissProt: P68433 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Histone H31 aa 1-50 / 136.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Immunology & Inflammation
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200
Molecular Weight
Predicted band size: 15 kDa
Subcellular location
Nucleus.
Positive control
F9 cell lysate, Hela, MCF-7, PC-3M, human liver tissue.