![AffiAB® Anti-Histone H3 Antibody [A11-D7]](/web/image/product.product/74655/image_1024/%5BAFG-HB-1868%5D%20AffiAB%C2%AE%20Anti-Histone%20H3%20Antibody%20%5BA11-D7%5D%20?unique=d31c23b)
AffiAB® Anti-Histone H3 Antibody [A11-D7]
The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4) , is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: P68431 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG2b
Clone
A11-D7
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
Zebrafish
Immunogen
Synthetic peptide within N-terminal human Histone H3.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Immunology & Inflammation
Recommended dilutions
WB: 1:5, 000-1:10, 000; IF-Cell: 1:200-1:500; IHC-P: 1:200-1:1, 000
Molecular Weight
Predicted band size: 15 kDa
Subcellular location
Nucleus.
Positive control
Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, F9 cell lysate, Hela, F9, rat testis tissue, human skin tissue, mouse brain tissue.