![AffiAB® Anti-Histone H3 Antibody [6-A7] - Nuclear Loading Control](/web/image/product.product/74654/image_1024/%5BAFG-HB-1867%5D%20AffiAB%C2%AE%20Anti-Histone%20H3%20Antibody%20%5B6-A7%5D%20-%20Nuclear%20Loading%20Control?unique=d31c23b)
AffiAB® Anti-Histone H3 Antibody [6-A7] - Nuclear Loading Control
The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4) , is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: P68431 Human; SwissProt: P68433 Mouse; SwissProt: Q6LED0 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG1
Clone
6-A7
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P, FC, IF-Cell, IF-Tissue
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Histone H3 aa 1-50 / 136.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Immunology & Inflammation
Recommended dilutions
WB: 1:10, 000-1:20, 000; IF-Cell: 1:200; IF-Tissue: 1:200; IHC-P: 1::400-1:800; FC: 1:500
Molecular Weight
Predicted band size: 15 kDa
Subcellular location
Nucleus.
Positive control
Hela cell lysates, NIH/3T3 cell lysates, PC12 cell lysates, Hela cells, human liver tissue, mouse testis tissue, rat brain tissue.