AffiAB® Anti-Histone H3 (acetyl K18) Antibody
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to methylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P68431 Human; SwissProt: P84243 Human; SwissProt: Q16695 Human; SwissProt: Q71DI3 Human; SwissProt: Q6NXT2 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human Histone H3 (acetyl K18) aa 1-50.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Immunology & Inflammation
Recommended dilutions
WB: 1:500-1:1, 000; IHC-P: 1:100-1:500
Molecular Weight
15 kDa
Subcellular location
Chromosome, Nucleosome core, Nucleus.
Positive control
Hela cell lysate , untreated, Hela cell lysate, treated with Sodium Butyrate 0.5mM for 24h, Hela cell lysate, treated with trichostatin at 500 ng/ml for 4h, mouse colon.