![AffiAB® Anti-Histone H3 (acetyl K14) Antibody [JU43-26]](/web/image/product.product/74636/image_1024/%5BAFG-HB-1848%5D%20AffiAB%C2%AE%20Anti-Histone%20H3%20%28acetyl%20K14%29%20Antibody%20%5BJU43-26%5D%20?unique=d31c23b)
AffiAB® Anti-Histone H3 (acetyl K14) Antibody [JU43-26]
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P68431 Human; SwissProt: P68433 Mouse; SwissProt: Q6LED0 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JU43-26
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, SNAP-ChIP, CUT&Tag-seq
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Histone H3 aa 1-50 / 136 (acetyl K14) .
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Immunology & Inflammation
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:200-1:1, 000; IF-Tissue: 1:200-1:1, 000; IHC-P: 1:50-1:200; SNAP-ChIP: 1:100; IP: Use at an assay dependent concentration.
Molecular Weight
15 kDa
Subcellular location
Nucleus. Chromosome.
Positive control
Jurkat cell lysate, rat liver tissue lysate, mouse lung tissue lysate, Hela, PC-3M, rat brain tissue, human kidney tissue, rat hippocampus tissue, human colon tissue.