![AffiAB® Anti-Histone H1.2 Antibody [JU43-48]](/web/image/product.product/74626/image_1024/%5BAFG-HB-1838%5D%20AffiAB%C2%AE%20Anti-Histone%20H1.2%20Antibody%20%5BJU43-48%5D%20?unique=d31c23b)
AffiAB® Anti-Histone H1.2 Antibody [JU43-48]
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P16403 Human; SwissProt: P15864 Mouse; SwissProt: P15865 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JU43-48
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, ChIP
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Histone H12 aa 1-50 / 213.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:100; IF-Tissue: 1:50-1:100; IHC-P: 1:50-1:200
Molecular Weight
21 kDa
Subcellular location
Nucleus. Chromosome.
Positive control
Hela cell lysate, 293 cell lysate, MCF-7 cell lysate, rat liver tissue lysate, mouse lung tissue lysate, Hela, PC-3M, SK-Br-3, mouse colon tissue, human kidney tissue, rat brain tissue, human lung carcinoma tissue.