AffiAB® Anti-Hexokinase II Antibody
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P52789 Human; SwissProt: O08528 Mouse; SwissProt: P27881 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human Hexokinase II aa 1-200.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:50-1:100; IHC-P: 1:100-1:500; FC: 1:500-1:1, 000
Molecular Weight
Predicted band size: 102 kDa
Subcellular location
Cytosol, Mitochondrion outer membrane.
Positive control
293 cell lysate, Jurkat cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, rat skeletal muscle tissue lysate, SiHa, rat testis tissue, human fetal skeletal muscle tissue, mouse kidney tissue, Hela.