AffiAB® Anti-HER2 / ErbB2 Antibody
HER2 is a member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family. Amplification or overexpression of this oncogene has been shown to play an important role in the development and progression of certain aggressive types of breast cancer. In recent years the protein has become an important biomarker and target of therapy for approx. 30% of breast cancer patients. Over-expression is also known to occur in ovarian, stomach, and aggressive forms of uterine cancer, such as uterine serous endometrial carcinoma. The expression of HER2 is regulated by signaling through estrogen receptors. Normally, estradiol and tamoxifen acting through the estrogen receptor down-regulate the expression of HER2. However, when the ratio of the coactivator AIB-3 exceeds that of the corepressor PAX2, the expression of HER2 is upregulated in the presence of tamoxifen, leading to tamoxifen-resistant breast cancer.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P04626 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human HER2 aa 1-50 / 1, 255.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
HIF-1 signaling pathway, PI3K-AKT, Cancer, Calcium signaling pathway, ErbB signaling pathway, Immunology & Inflammation
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:50-1:100; IHC-P: 1:50-1:1, 000
Molecular Weight
Predicted band size: 138 kDa
Subcellular location
Cell membrane.
Positive control
SK-Br-3 cell lysate, SKOV-3 cell lysate, SKOV-3, human stomach carcinoma tissue, human breast carcinoma tissue, human placenta tissue.