AffiAB® Anti-HEC1 Antibody
Kinetochore protein NDC80 homolog is a protein that in humans is encoded by the NDC80 gene. Ndc80 is one of the proteins of outer kinetochore. It forms a heterotetramer with proteins NUF2, SPC25, and SPC24. This protein complex has microtubule-binding domains. HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: O14777 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human HEC1 aa 300-505 / 642.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500
; FC: 1:50-1:100
Molecular Weight
Predicted band size: 74 kDa
Subcellular location
Nucleus.
Positive control
SiHa cell lysates, HL-60 cell lysates, MG-63.