AffiAB® Anti-HDGF Antibody [JE56-32]
This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P51858 Human; SwissProt: P51859 Mouse; SwissProt: Q8VHK7 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JE56-32
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human HDGF aa 50-200.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Cancer
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:50-1:100; IHC-P: 1:200-1:1, 000; FC: 1:50-1:100
Molecular Weight
Predicted band size: 27 kDa.
Subcellular location
Nucleus, Cytoplasm, Extracellular exosome.
Positive control
Rat cerebellum tissue lysate, SK-Br-3 cell lysate, A549 cell lysate, mouse cerebellum tissue lysate, F9, human liver carcinoma tissue, human cervix tissue, human colon carcinoma tissue, human breast carcinoma tissue, human esophagus tissue, human stomach carcinoma tissue, human colon tissue, THP-1.