![AffiAB® Anti-HDAC8 Antibody [JJ0845]](/web/image/product.product/74589/image_1024/%5BAFG-HB-1799%5D%20AffiAB%C2%AE%20Anti-HDAC8%20Antibody%20%5BJJ0845%5D%20?unique=a48b62f)
AffiAB® Anti-HDAC8 Antibody [JJ0845]
Histone deacetylase 8 is an enzyme that in humans is encoded by the HDAC8 gene. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation / deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. Histone deacetylase 8 is involved in skull morphogenesis and metabolic control of the ERR-alpha / PGC1-alpha transcriptional complex. HDAC8 has been linked to number of disease states notably to acute myeloid leukemia and is related to actin cytoskeleton in smooth muscle cells. siRNA targeting HDAC8 showed anticancer effects. Inhibition of HDAC8 induced apoptosis has been observed in T cell lymphomas. In addition the HDAC8 enzyme has been implicated in the pathogenesis of neuroblastoma.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q9BY41 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JJ0845
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human HDAC8 aa 51-100 / 377.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Immunology & Inflammation, Cardiovascular
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:100-1:500; IF-Tissue: 1:100-1:500; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 42 kDa
Subcellular location
Nucleus, Cytoplasm.
Positive control
Hela cell lysate, K562 cell lysate, Hela, A549, HepG2, human lung carcinoma tissue, human kidney tissue, human pancreas tissue, human lung tissue, K562.