AffiAB® Anti-GST Antibody [SN70-05]
The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
N/A
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SN70-05
KO/KD
N/A
Species reactivity
N/A
Tested applications
WB, IF-Cell, IP
Predicted species reactivity
N/A
Immunogen
GST tag recombinant protein.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:5, 000; IF-Cell: 1:50-1:200; IP: Use at an assay dependent concentration.
Molecular Weight
N/A
Subcellular location
N/A
Positive control
GST tag recombinant protein