AffiAB® Anti-GST Antibody [16-21]
The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
N/A
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG1
Clone
16-21
KO/KD
N/A
Species reactivity
N/A
Tested applications
WB, ELISA
Predicted species reactivity
N/A
Immunogen
recombinant protein.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:5, 000
; ELISA: 1:10, 000-1:20, 000
Molecular Weight
N/A
Subcellular location
N/A
Positive control
GST-tagged recombinant protein.