AffiAB® Anti-GST Antibody
The pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Anti-bodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots, as well as for immuno affinity purification of proteins expressed in E. coli.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
N/A
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
N/A
Tested applications
WB, ELISA
Predicted species reactivity
N/A
Immunogen
Recombinant protein within GST protein.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:10, 000-1:100, 000
; ELISA: 1:10, 000
Molecular Weight
N/A
Subcellular location
N/A
Positive control
GST fusion-protein