AffiAB® Anti-GSDMC Antibody
This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-C, N-terminal) binds to membranes and forms pores, triggering cell death. Pore-forming protein that causes membrane permeabilization and pyroptosis. Released upon cleavage and binds to membrane inner leaflet lipids. Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis. The functional mechanisms and physiological proteases that cleave and activate this pore-forming protein are unknown.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q9BYG8 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human GSDMC aa 1-220 / 508.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Cancer
Recommended dilutions
WB:1:1, 000-1:2, 000
; IHC-P:1:50-1:200
; FC:1:50-1:100
Molecular Weight
Predicted band size: 58 kDa
Subcellular location
Cell membrane, Cytoplasm, Membrane.
Positive control
Human stomach tissue lysates, human lung tissue, human kidney tissue, A549.