AffiAB® Anti-GSDMA Antibody
May promote pyroptosis (Probable) . Upon cleavage in vitro of genetically engineered GSDMA, the released N-terminal moiety binds to some types of lipids, such as possibly phosphatidylinositol (4, 5) - bisphosphate. Homooligomerizes within the membrane and forms pores of 10 -15 nanometers (nm) of inner diameter, triggering cell death. Also binds to bacterial and mitochondrial lipids, including cardiolipin, and exhibits bactericidal activity. The physiological relevance of these observations is unknown (Probable) .
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q96QA5 Human; Entrez Gene: 360619 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Rat
Tested applications
WB
Predicted species reactivity
N/A
Immunogen
Recombinant protein within human GSDMA aa 1-200.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:2, 000
Molecular Weight
Predicted band size: 49 kDa.
Subcellular location
Cytosol, perinuclear region; Cell membrane.
Positive control
Human skin tissue lysate, rat skin tissue lysate.