AffiAB® Anti-Grp75 Antibody
This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P38646 Human; SwissProt: P38647 Mouse; SwissProt: P48721 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within C-terminal human Grp75.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Cancer
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:100; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
Predicted band size: 74 kDa
Subcellular location
Mitochondrion, nucleolus.
Positive control
PC-12 cell lysate, MCF-7 cell lysate, Hela cell lysate, NIH/3T3 cell lysate, mouse kidney tissue lysate, mouse brain tissue lysate, HepG2, MCF-7, N2A, SH-SY5Y, mouse testis tissue, mouse brain tissue, mouse prostate tissue.