![AffiAB® Anti-GRAMD1A Antibody [1-E4]](/web/image/product.product/74526/image_1024/%5BAFG-HB-1732%5D%20AffiAB%C2%AE%20Anti-GRAMD1A%20Antibody%20%5B1-E4%5D%20?unique=315d7a9)
AffiAB® Anti-GRAMD1A Antibody [1-E4]
GRAMD1A first finds in human embryonic stem cell, and expresses in ectoderm, mesoderm and endoderm tissues and many tumor cells, its function hasn’t been explored. The researh has determined the role of GRAM1DA in the self-renewal of HCC stem cell and resistance to chemotherapy by overexpressing GRAMD1A, and the role of GRAMD1A in tumor growth by overexpressing or downregulating GRAMD1A. GRAMD1A is a prognostic factor for HCC, it promoted the self-renewal of HCC stem cell, resistance to chemotherapy and tumor growth through promoting STAT5, Inhibition of STAT5 in indicated cells with GRAMD1A overexpression inhibited the self-renewal of HCC stem cell, resistance to chemotherapy and tumor growth.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: Q96CP6 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgM
Clone
1-E4
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human GRAMD1A aa 1-724 / 724.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:2, 000; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:400
Molecular Weight
Predicted band size: 81 kDa
Subcellular location
Endoplasmic reticulum membrane, Cell membrane, Cytoplasmic vesicle, autophagosome.
Positive control
NIH/3T3 cells lysates, SHG-44 cells lysates, A549, human liver tissue, human liver carcinoma tissue.