AffiAB® Anti-Goat IgG Light chain Antibody [B8-C4-G4]
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.TRITC (tetraethyl rhodamine isothiocyanate) is the form of fluorescein used for conjugation to all of our antibodies and purified proteins, with the exception of streptavidin. This monoclonal antibody can be used to detect the light chain of goat immunoglobulin.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
N/A
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG2b
Clone
B8-C4-G4
KO/KD
N/A
Species reactivity
Goat
Tested applications
WB, ELISA, IP
Predicted species reactivity
N/A
Immunogen
Native protein.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein G affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000-1:2, 000
; ELISA: 1:2, 000-1:5, 000
; IP: 1:100-1:500
Molecular Weight
25 kDa
Subcellular location
N/A
Positive control
Goat IgG protein lysates.