AffiAB® Anti-GLO1 Antibody [JU44-11]
The glyoxal pathway plays a role in the detoxification of glucose degradation products (GDP) . Glyoxalase I (GLO1) , a member of the glyoxalase family, is effective in eliminating GDP. Overexpression or silencing of Glyoxalase I in mice brain suggests an association between Glyoxalase I and anxiety. Glyoxalase I has three isoforms generated from two alleles in the genome which forms two homodimers and one heterodimer, each subunit binding one zinc ion. Research demonstrates that GLO1 gene expression is induced in colon carcinoma. Both an insulin response element (IRE) , and a zinc metal response element (MRE) in the promoter region of the GLO1 gene have been identified.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q04760 Human; SwissProt: SwissProt: Q9CPU0 Mouse; SwissProt: SwissProt: Q6P7Q4 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JU44-11
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human GLO1 aa 1-120 / 184.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:100-1:500; IHC-P: 1:100-1:500; FC: 1:50-1:100
Molecular Weight
21 kDa
Subcellular location
Cytoplasm. Nucleus.
Positive control
Mouse testis tissue lysate, C2C12 cell lysate, 293 cell lysate, rat spleen tissue lysate, 293T, HUVEC, SH-SY5Y, rat epididymis tissue, human thyroid tissue, Hela.