AffiAB® Anti-GIT1 Antibody [JU33-39]

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CAT# AFG-HB-1647
Size: 100 μL

496.50 496.5 USD 496.50

496.50

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AffiAB® Anti-GIT1 Antibody [JU33-39]

Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. G protein-coupled receptor kinases (GRKs) are activated by activated G protein-coupled receptors, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade. GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, which are involved in vesicular trafficking. GIT1 overexpression results in reduced internalization and resensitization of b2-adrenergic receptor, thus reducing b2-adrenergic receptor signaling.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: Q9Y2X7 Human; SwissProt: Q68FF6 Mouse; SwissProt: Q9Z272 Rat

Recombinant

YES

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

JU33-39

KO/KD

N/A

Species reactivity

Human, Mouse, Rat

Tested applications

WB, IHC-P, FC

Predicted species reactivity

N/A

Immunogen

Recombinant protein within Human GIT1 aa 79-297 .

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

N/A

Recommended dilutions

WB: 1:500-1:1, 000 ; IHC-P: 1:50-1:400 ; FC: 1:50-1:100

Molecular Weight

Predicted band size: 84 kDa

Subcellular location

Cytoplasm.

Positive control

A549 cell lysates, human colon carcinoma tissue, human pancreas tissue, mouse fallopian tubes, rat brain tissue, A549, mouse small intestine tissue.