![AffiAB® Anti-GFP Antibody [SR48-02]](/web/image/product.product/74442/image_1024/%5BAFG-HB-1644%5D%20AffiAB%C2%AE%20Anti-GFP%20Antibody%20%5BSR48-02%5D%20?unique=d31c23b)
AffiAB® Anti-GFP Antibody [SR48-02]
Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm) . GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P42212 Aequorea victoria
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SR48-02
KO/KD
N/A
Species reactivity
N/A
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Aequorea victoria GFP aa 1-50 / 238.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:5, 000-1:10, 000; IHC-P:1:200-1:500; IF-Cell: 1:100-1:500; IF-Tissue: 1:100-1:500; IP: 2-5 µg/ml
Molecular Weight
Predicted band size: 27 kDa
Subcellular location
N/A
Positive control
Recombinant GFP protein, GFP Tag fusion protein lysate.