AffiAB® Anti-GFP Antibody [SP00-68]

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CAT# AFG-HB-1642
Size: 100 μL

346.50 346.5 USD 346.50

346.50

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AffiAB® Anti-GFP Antibody [SP00-68]

The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: P42212 AequoreaVictoria

Recombinant

YES

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

SP00-68

KO/KD

N/A

Species reactivity

N/A

Tested applications

WB, IHC-P, IF-Cell, IF-Tissue, IP

Predicted species reactivity

N/A

Immunogen

Synthetic peptide within Aequorea victoria GFP aa 1-50 / 238.

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

N/A

Recommended dilutions

WB: 1:10, 000-1:50, 000; IF-Cell: 1:500-1:1, 000; IF-Tissue: 1:500-1:1, 000; IHC-P: 1:500-1:1, 000; IP: 2-5 µg/ml.

Molecular Weight

27 kDa

Subcellular location

N/A

Positive control

K562 cell lysates transfected with GFP.