AffiAB® Anti-GFP Antibody [B2-F7-C10]

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CAT# AFG-HB-1641
Size: 100 μL

315.00 315.0 USD 315.00

315.00

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AffiAB® Anti-GFP Antibody [B2-F7-C10]

The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.

Antibody type

Mouse monoclonal Antibody

Uniprot ID

SwissProt: P42212 AequoreaVictoria

Recombinant

NO

Conjugation

Non-conjugated

Host

Mouse

Isotype

IgG

Clone

B2-F7-C10

KO/KD

N/A

Species reactivity

N/A

Tested applications

WB

Predicted species reactivity

N/A

Immunogen

Recombinant full length protein of Aequorea victoria GFP.

Storage

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 mg/mL.

Purity

Protein G affinity purified.

Signal pathway

N/A

Recommended dilutions

WB: 1:1, 000-1:5, 000

Molecular Weight

26 kDa

Subcellular location

N/A

Positive control

K562 cell lysates transfected with GFP.