AffiAB® Anti-GFP Antibody [A11-C11]
Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm) . GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: P42212 AEQVI
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG2b
Clone
A11-C11
KO/KD
N/A
Species reactivity
N/A
Tested applications
IF-Cell, IHC-P, IP
Predicted species reactivity
N/A
Immunogen
Recombinant full length protein of Aequorea victoria GFP.
Storage
Store at -20°C. Stable for 12 months from date of receipt.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein G affinity purified.
Signal pathway
N/A
Recommended dilutions
IF-Cell:1:500-1:1, 000; IHC-P: 1:100; IP: 2-5 µg/ml.
Molecular Weight
Predicted band size: 27 kDa
Subcellular location
Secreted.
Positive control
K562 cells transfected with GFP