AffiAB® Anti-GFP Antibody
Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm) . GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P42212 Aequorea victoria
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
N/A
Tested applications
WB, ELISA, IP
Predicted species reactivity
N/A
Immunogen
Recombinant full length protein of Aequorea victoria GFP.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:10, 000
; ELISA: 1:10, 000
; IP: 2-5 µg/ml.
Molecular Weight
Predicted band size: 26 kDa
Subcellular location
N/A
Positive control
GFP protein