AffiAB® Anti-GFAP Antibody
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. In addition, GFAP intermediate filaments are also present in nonmyelin-forming Schwann cells in the peripheral nervous system.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P14136 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within C-terminal residues of GFAP.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
JAK-STAT signaling pathway, Neuroscience
Recommended dilutions
WB: 1:5, 000-1:10, 000; IF-Cell: 1:50-1:100; IHC-P: 1:200
Molecular Weight
Predicted band size: 50 kDa
Subcellular location
Cytoplasm, Intermediate filament.
Positive control
Human brain, A172, mouse brain tissue lysate, rat brain tissue lysate.