AffiAB® Anti-Geminin Antibody
Geminin is a nuclear protein that is present in most eukaryotics and highly conserved across species. Numerous functions have been elucidated for Geminin including roles in metazoan cell cycle, cellular proliferation, cell lineage commitment, and neural differentiation. During S phase, geminin is a negative regulator of DNA replication. In many cancer cell lines, inhibition of geminin by RNAi results in re-replication of portions of the genome, which leads to aneuploidy.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: O75496 Human; SwissProt: O88513 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, FC
Predicted species reactivity
Rat
Immunogen
Synthetic peptide within Human Geminin aa 1-50 / 209.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500; IF-Cell: 1:50; FC: 1:50-1:100
Molecular Weight
24 kDa
Subcellular location
Nucleus, cytoplasm
Positive control
Hela cell lysates, NCCIT, F9, HepG2.