AffiAB® Anti-Gastrin Antibody [PD00-37]
Gastrin is a peptide hormone that stimulates secretion of gastric acid (HCl) by the parietal cells of the stomach and aids in gastric motility. It is released by G cells in the pyloric antrum of the stomach, duodenum, and the pancreas. Gastrin binds to cholecystokinin B receptors to stimulate the release of histamines in enterochromaffin-like cells, and it induces the insertion of K+/H+ ATPase pumps into the apical membrane of parietal cells (which in turn increases H+ release into the stomach cavity) . Its release is stimulated by peptides in the lumen of the stomach. In the Zollinger–Ellison syndrome, gastrin is produced at excessive levels, often by a gastrinoma gastrin-producing tumor, mostly benign of the duodenum or the pancreas. In autoimmune gastritis, the immune system attacks the parietal cells leading to hypochlorhydria low stomach acid secretion. This results in an elevated gastrin level in an attempt to compensate for increased pH in the stomach. Eventually, all the parietal cells are lost and achlorhydria results leading to a loss of negative feedback on gastrin secretion. Plasma gastrin concentration is elevated in virtually all individuals with mucolipidosis type IV (mean 1507 pg/mL; range 400-4100 pg/mL) (normal 0-200 pg/mL) secondary to a constitutive achlorhydria. This finding facilitates the diagnosis of patients with this neurogenetic disorder. Additionally, elevated gastrin levels may be present in chronic gastritis resulting from H pylori infection.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P01350 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
PD00-37
KO/KD
N/A
Species reactivity
Human
Tested applications
IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Gastrin (internal sequence) .
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
PBS (pH7.4) , 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
IHC-P: 1:1, 000
Molecular Weight
Predicted band size: 11 kDa
Subcellular location
Secreted.
Positive control
Human stomach tissue.