![AffiAB® Anti-GAPDH Antibody [5-E10]](/web/image/product.product/74396/image_1024/%5BAFG-HB-1593%5D%20AffiAB%C2%AE%20Anti-GAPDH%20Antibody%20%5B5-E10%5D%20?unique=8544d55)
AffiAB® Anti-GAPDH Antibody [5-E10]
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: P04406 Human; SwissProt: P16858 Mouse; SwissProt: P04797 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgM
Clone
5-E10
KO/KD
N/A
Species reactivity
Human, Mouse, Rat, Zebrafish, Rabbit
Tested applications
WB, IF-Cell
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human GAPDH aa 10-50.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein L affinity purified.
Signal pathway
HIF-1 signaling pathway, Neuroscience, Alzheimer disease
Recommended dilutions
WB: 1:5, 000-1:10, 000; IF-Cell: 1:200-1:500
Molecular Weight
Predicted band size: 36 kDa
Subcellular location
Cytoplasm
Positive control
HepG2 cell lysate, Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, MCF-7 cell lysate, rabbit liver tissue lysate, zebrafish tissue lysate, F9, Hela, HepG2.