AffiAB® Anti-GAP43 Antibody
GAP43, is a nervous tissue-specific cytoplasmic protein that can be attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4. This sequence targets GAP43 to lipid rafts. It is a major protein kinase C (PKC) substrate and is considered to play a key role in neurite formation, regeneration, and plasticity. The role of GAP-43 in CNS development is not limited to effects on axons: It is also a component of the centrosome, and differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. As a consequence, in the cerebellum, the neuronal precursor pool fails to expand normally and the cerebellum is significantly smaller.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P17677 Human; SwissProt: P06837 Mouse; SwissProt: P07936 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within mouse GAP43 aa 178-227 / 227.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:1, 000; IF-Cell: 1:200; IHC-P: 1:200; FC: 1:100-1:200
Molecular Weight
Predicted band size: 25 kDa
Subcellular location
Cell membrane, Cell projection, Cytoplasm, Membrane, Synapse.
Positive control
Rat brain tissue lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Human skeletal muscle tissue lysate, N2A cell lysate, A172 cell lysate, Human heart tissue lysate, SHG-44, rat brain tissue, mouse brain tissue.