AffiAB® Anti-GADD34 Antibody
Protein phosphatase 1 regulatory subunit 15A also known as growth arrest and DNA damage-inducible protein GADD34 is a protein that in humans is encoded by the PPP1R15A gene. The Gadd34/MyD116 gene was originally discovered as a member in a set of gadd and MyD mammalian genes encoding acidic proteins that synergistically suppress cell growth. Later on it has been characterized as a gene playing a role in ER stress-induced cell death, being a target of ATF4 that plays a role in ER-mediated cell death via promoting protein dephosphorylation of eIF2α and reversing translational inhibition. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: O75807 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human GADD34 aa 1-50.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:1, 000; IHC-P: 1:50-1:1200; FC: 1:50-1:100
Molecular Weight
Predicted band size: 73 kDa.
Subcellular location
Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane.
Positive control
HepG2 cell lysate, A431 cell lysate, human skin tissue, human prostate carcinoma tissue, human placenta tissue, K562.