![AffiAB® Anti-Fructose 6 Phosphate Kinase Antibody [JU53-31]](/web/image/product.product/74340/image_1024/%5BAFG-HB-1525%5D%20AffiAB%C2%AE%20Anti-Fructose%206%20Phosphate%20Kinase%20Antibody%20%5BJU53-31%5D%20?unique=a48b62f)
AffiAB® Anti-Fructose 6 Phosphate Kinase Antibody [JU53-31]
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1, 6-bisphosphate by ATP, the first committing step of glycolysis. In human PFK exists as a system of 3 types of subunits, PFKM (muscle) , PFKL (liver) and PFKP (platelet) isoenzymes.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P08237 Human; SwissProt: P47857 Mouse; SwissProt: P47858 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JU53-31
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Fructose 6 Phosphate Kinase aa 107-156 / 780.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
HIF-1 signaling pathway, AMPK signaling pathway
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
85 kDa
Subcellular location
Cytoplasm.
Positive control
HepG2 cell lysate, PC-3M cell lysate, LOVO, PC-3M, SH-SY5Y, rat brain tissue, human liver carcinoma tissue, mouse skeletal muscle tissue, human thyroid tissue, human kidney tissue.