AffiAB® Anti-Frizzled-5 Antibody
Frizzled-5 belongs to the G-protein coupled receptor Fz/Smo family, contains 585 amino acids. It is localized at the plasma membrane and also found at the Golgi. Fzds have a large extracellular N-terminal region containing a cysteine-rich domain (CRD) , which is involved in binding to Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q13467 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Frizzled-5 aa 143-192 / 585.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
Alzheimer disease
Recommended dilutions
WB:1:500-1:1, 000; IF-Cell:1:50-1:200; FC:1:50-1:100
Molecular Weight
Predicted band size: 64 kDa
Subcellular location
Cell membrane.Golgi apparatus. Membrane. Synapse.
Positive control
Human ES cell, A549, LOVO, HepG2.