AffiAB® Anti-FOXA1 Antibody [JF10-02]
Forkhead box protein A1 (FOXA1) , also known as hepatocyte nuclear factor 3-alpha (HNF-3A) , is a protein that in humans is encoded by the FOXA1 gene. FOXA1 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin as a pioneer factor. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. Mutations in this gene have been recurrently seen in instances of prostate cancer. Expression of FOXA1 correlates with two EMT markers, namely Twist1 and E-cadherin in breast cancer.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P55317 Human; SwissProt: P35582 Mouse; SwissProt: P23512 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JF10-02
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human FOXA1 aa 320-472 / 472.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200
Molecular Weight
Predicted band size: 49 kDa
Subcellular location
Nucleus.
Positive control
SK-Br-3 cell lysate, MCF-7 cell lysate, MCF-7, rat prostate tissue, human breast tissue, mouse colon tissue, human breast carcinoma tissue.