![AffiAB® Anti-Firefly Luciferase Antibody [SR03-86]](/web/image/product.product/74300/image_1024/%5BAFG-HB-1481%5D%20AffiAB%C2%AE%20Anti-Firefly%20Luciferase%20Antibody%20%5BSR03-86%5D%20?unique=a48b62f)
AffiAB® Anti-Firefly Luciferase Antibody [SR03-86]
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P08659 Firefly
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SR03-86
KO/KD
N/A
Species reactivity
Firefly
Tested applications
WB, IF-Cell, IF-Tissue
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Firefly Luciferase aa 132-243 / 550.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000
Molecular Weight
Predicted band size: 61 kDa
Subcellular location
Peroxisome.
Positive control
Luciferase recombinant protein.