AffiAB® Anti-FEN1 Antibody
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P39748 Human; SwissProt: P39749 Mouse; SwissProt: Q5XIP6 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P, IF-Cell, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human FEN1 aa 170-380 / 380.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/ml.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IHC-P: 1:50-1:200; IF-Cell: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
Predicted band size: 42 kDa
Subcellular location
Mitochondrion, Nucleus.
Positive control
WB: K562 cell lysate, Daudi cell lysate, SHSY5Y cell lysate. ICC: MCF-7 cell, SHSY5Y cell. IHC: Human tonsil tissue, human lung cancer tissue, human colon tissue, mouse testis tissue. FC: MG-63 cell.