AffiAB® Anti-ERp72 Antibody [10-F2]
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly.
Antibody type
Mouse monoclonal Antibody
Uniprot ID
SwissProt: P13667 Human; SwissProt: P08003 Mouse; SwissProt: P38659 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Mouse
Isotype
IgG1
Clone
10-F2
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P, IF-Cell, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human ERp72 aa 596-645 / 645.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 mg/mL.
Purity
Protein G affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:50; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
Predicted band size: 73 kDa
Subcellular location
Endoplasmic reticulum.
Positive control
A431 cell lysates, HepG2, SKOV-3, rat testis tissue, human lung cancer tissue, human liver tissue, human placenta tissue, mouse brain tissue, SiHa.