AffiAB® Anti-ERp29 Antibody [JG34-42]
ERp29 is a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER) . The protein shows sequence similarity to the protein disulfide-isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P30040 Human; SwissProt: P57759 Mouse; SwissProt: P52555 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JG34-42
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IHC-P, IF-Cell, IF-Tissue
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human ERp29 aa 1-142 / 261.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200
Molecular Weight
Predicted band size: 29 kDa
Subcellular location
Endoplasmic reticulum.
Positive control
THP-1 cell lysate, F9, PMVEC, SiHa, rat testis tissue, human thyroid gland tissue, human colon cancer tissue.