AffiAB® Anti-ERK2 Antibody
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P28482 Human; SwissProt: P63085 Mouse; SwissProt: P63086 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within C-terminal human ERK2.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
VEGF signaling pathway, HIF-1 signaling pathway, PI3K-AKT, 衰老, Type II diabetes mellitus, cAMP signaling pathway, ErbB signaling pathway, RAS signaling pathway, Immunology & Inflammation, Autophagy Alzheimer disease, MAPK signaling pathway, cGMP-PKG signaling pathway
Recommended dilutions
WB:1:1, 000-1:2, 000; IF-Cell:1:200; IHC-P:1:200; FC:1:100-1:200
Molecular Weight
Predicted band size: 41 kDa
Subcellular location
Cytoplasm, Nucleus, Membrane, Cytoskeleton.
Positive control
Jurkat cell lysate, A431 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, Hela cell lysate, HT-29 cell lysate, MCF-7 cell lysate, A549, human breast cancer tissue, human kidney tissue, mouse kidney tissue.