AffiAB® Anti-ERK1 Antibody
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines , and research investigators consider it an important target in the diagnosis and treatment of cancer . Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K) , a MAP kinase kinase (MAPKK or MAP2K) , and a MAP kinase (MAPK) . Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P27361 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within human ERK1 aa 306-360.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
VEGF signaling pathway, HIF-1 signaling pathway, Neuroscience, PI3K-AKT, 衰老, Type II diabetes mellitus, cAMP signaling pathway, ErbB signaling pathway, RAS signaling pathway, Immunology & Inflammation, Autophagy Alzheimer disease, MAPK signaling pathway, cGMP-PKG signaling pathway
Recommended dilutions
WB: 1:500-1:5, 000; IF-Cell: 1:200; IHC-P: 1:200
Molecular Weight
Predicted band size: 43 kDa
Subcellular location
Cytoplasm, Nucleus, Cell junction, Membrane.
Positive control
Hela cell lysate, SW480 cell lysate, HCT116 cell lysate, HepG2 cell lysate, PC12 cell lysate, F9 cell lysate, LOVO, human colon carcinoma tissue, human breast carcinoma tissue, mouse large intestine tissue.