AffiAB® Anti-ERG Antibody
Transcriptional regulator ERG is a nuclear protein that binds purine-rich sequences of DNA. Transcriptional regulator ERG is required for platelet adhesion to the subendothelium and regulates hematopoiesis. It has a DNA binding domain and a PNT (pointed) domain. ERG is expressed at higher levels in early myelocytes than in mature lymphocytes (types of white blood cells) . Therefore ERG may act as a regulator of differentiation of early hematopoietic cells. This gene can be classified as a proto-oncogene, which participates in chromosomal translocations (a popular example of this phenomenon is the Philadelphia chromosome) . Essentially during cell division ERG can accidentally get stuck onto a different chromosome than where it belongs.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P11308 Human; SwissProt: P81270 Mouse
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within C-terminalhuman ERG.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500; IF-Cell: 1:200
Molecular Weight
Predicted band size: 54 kDa
Subcellular location
Nucleus, cytoplasm
Positive control
Jurkat, MCF-7, PANC-1