![AffiAB® Anti-Epac1 Antibody [JM63-29]](/web/image/product.product/74168/image_1024/%5BAFG-HB-1339%5D%20AffiAB%C2%AE%20Anti-Epac1%20Antibody%20%5BJM63-29%5D%20?unique=a48b62f)
AffiAB® Anti-Epac1 Antibody [JM63-29]
3', 5' cyclic adenosine monophosphate (cAMP) -regulated guanine nucleotide exchange factors Epac (Epac1, cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the Ras family GTPases Rap 1 and Rap 2 by promoting GTP binding in a cAMP-dependent manner. Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission. The human Epac gene maps to chromosome 12q13.11 with transcript being abundant in the kidney and heart. In situ hybridization indicates expression of Epac in adult rat brain and selective expression in neonatal brain, including septum and thalamus.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: O95398 Human; SwissProt: Q8VCC8 Mouse
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JM63-29
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IHC-P, IP, IF-Cell, IF-Tissue
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human Epac1 aa 874-923 / 923.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
cAMP signaling pathway
Recommended dilutions
WB: 1:500-1:1, 000; IHC-P: 1:50-1:200; IP: 1:10-1:50; IF-Cell: 1:10-1:50; IF-Tissue: 1:10-1:50
Molecular Weight
Predicted band size: 104 kDa
Subcellular location
Endomembrane system.
Positive control
Mouse kidney tissue lysates, PC-3 cell lysates, human prostate tissue, human stomach carcinoma tissue, mouse kidney tissue.