AffiAB® Anti-eNOS Antibody
Endothelial NOS (eNOS) , also known as nitric oxide synthase 3 (NOS3) , generates NO in blood vessels and is involved with regulating vascular function. The gene coding for eNOS is located on Chromosome 7. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with "caveolae" a component of plasma membranes surrounding cells, and the membranes of Golgi bodies within cells. eNOS localisation to endothelial membranes is mediated by cotranslational N-terminal myristoylation and post-translational palmitoylation.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P29474 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
IHC-P
Predicted species reactivity
Rat
Immunogen
Synthetic peptide within C-terminal human eNOS.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
VEGF signaling pathway, HIF-1 signaling pathway, PI3K-AKT, Calcium signaling pathway, cGMP-PKG signaling pathway
Recommended dilutions
IHC-P: 1:50-1:100
Molecular Weight
N/A
Subcellular location
Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane.
Positive control
Mouse testis tissue, mouse kidney tissue, human liver carcinoma tissue, human mammary gland.