AffiAB® Anti-ENO1 Antibody [JM63-53]
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P06733 Human; SwissProt: P17182 Mouse; SwissProt: P04764 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JM63-53
KO/KD
N/A
Species reactivity
Human, Mouse, Rat, Zebrafish
Tested applications
WB, FC, IF-Cell, IF-Tissue, IP
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human ENO1 aa 1-50 / 434.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; FC: 1:50-1:100; IF-Cell: 1:10-1:50; IF-Tissue: 1:10-1:50; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 47 kDa
Subcellular location
Cell membrane, Cytoplasm, Membrane, Nucleus.
Positive control
Rat brain tissue lysate, NIH-3T3 cell lysate, Zebrafish tissue lysates, HepG2 cell lysate, Hela cell lysate, MCF-7.